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Nacalai f 12
F 12, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/f+12/12+f/pm42319542-23-24-25
Average 86 stars, based on 1 article reviews
f 12 - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Cell Culture:

Article Title: Dapagliflozin confers protection against ferroptosis in cardiomyocyte via the inhibition of interferon-gamma pathway.
Article Snippet: .. HL-1, mouse cardiomyocytes (JCRB Cell Bank, Tokyo, Japan), were cultured on gelatin (0.1%)-fibronectin (1 mg/ mL)-coated culture plates, in modified Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 (Nacalai Tesque, Inc., Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (Sigma-Aldrich, St. Louis, USA) and 1% antibiotic cocktail mixture (Nacalai Tesque, Inc., Kyoto, Japan) under standard culture condition (5% CO2 at 37 °C). ..

Article Title: Differentiation of mesenchymal stem/stromal cells into CD45 + macrophage-like cells: expanding insights into MSC plasticity
Article Snippet: Before staining, the red blood cells were lysed using water (Nacalai Tesque, Kyoto, Japan). .. Bone marrow cells were cultured overnight on 6 cm FNC-coated (Athena Enzyme Systems, Baltimore, MD, USA) plastic dishes in DMEM/Ham’s F-12 (Nacalai, Kyoto, Japan). ..

Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
Article Snippet: .. HEK293T (ATCC, #CRL-3216) and hTERT-RPE1 (ATCC, #CRL-4000) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Nacalai Tesque) or DMEM/Ham’s F-12 (Nacalai Tesque) supplemented with 10% fetal bovine serum (Japan Bioserum) and 100 U/mL penicillin-streptomycin solution (Thermo Fisher Scientific), and maintained under 5% CO2 at 37°C. .. For experiments involving doxycycline induction, eMAT-inducible cells were cultured in medium supplemented with 10% Tet System Approved FBS (Clontech, #631101) instead of the standard fetal bovine serum.

Modification:

Article Title: Dapagliflozin confers protection against ferroptosis in cardiomyocyte via the inhibition of interferon-gamma pathway.
Article Snippet: .. HL-1, mouse cardiomyocytes (JCRB Cell Bank, Tokyo, Japan), were cultured on gelatin (0.1%)-fibronectin (1 mg/ mL)-coated culture plates, in modified Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 (Nacalai Tesque, Inc., Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (Sigma-Aldrich, St. Louis, USA) and 1% antibiotic cocktail mixture (Nacalai Tesque, Inc., Kyoto, Japan) under standard culture condition (5% CO2 at 37 °C). ..

Article Title: Development of Freeze-Dried Hyaluronic Acid Sheets for Healing Oral Mucositis: Influence of Hyaluronic Acid Molecular Weight and Nicotinamide Mononucleotide Loading on Healing Efficacy.
Article Snippet: Ammonium formate and methanol (HPLC grade) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). .. Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 and Triton X-100 were also purchased from Nacalai Tesque, Inc. https://doi.org/10.3390/jfb17030137 (Kyoto, Japan). .. Fetal bovine serum and penicillin–streptomycin were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Development of Freeze-Dried Hyaluronic Acid Sheets for Healing Oral Mucositis: Influence of Hyaluronic Acid Molecular Weight and Nicotinamide Mononucleotide Loading on Healing Efficacy
Article Snippet: Ammonium formate and methanol (HPLC grade) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). .. Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 and Triton X-100 were also purchased from Nacalai Tesque, Inc. (Kyoto, Japan). .. Fetal bovine serum and penicillin–streptomycin were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence
Article Snippet: .. HEK293T (ATCC, #CRL-3216) and hTERT-RPE1 (ATCC, #CRL-4000) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Nacalai Tesque) or DMEM/Ham’s F-12 (Nacalai Tesque) supplemented with 10% fetal bovine serum (Japan Bioserum) and 100 U/mL penicillin-streptomycin solution (Thermo Fisher Scientific), and maintained under 5% CO2 at 37°C. .. For experiments involving doxycycline induction, eMAT-inducible cells were cultured in medium supplemented with 10% Tet System Approved FBS (Clontech, #631101) instead of the standard fetal bovine serum.

Knock-Out:

Article Title: Galectin-3 in endometrial small extracellular vesicles promotes cytotrophoblast cell fusion during the implantation phase.
Article Snippet: .. The ST medium consisted of DMEM/Ham’s F-12 (#11581-15, Nacalai Tesque) supplemented with 0.1 mM 2-mercaptoethanol, 0.3% bovine serum albumin (BSA; #01863-77, Nacalai Tesque), 1% insulin–transferrin– selenium–ethanolamine (ITS-X supplement; #094-06761, FUJIFILM Wako), 4% serum replacement (KnockOut Serum Replacement; #10828010, Thermo Fisher Scientific), 2 μM forskolin (#11018, Cayman Chemical), and 2.5 mM Y-27632, as previously described [26]. ..

Article Title: Galectin-3 in endometrial small extracellular vesicles promotes cytotrophoblast cell fusion during the implantation phase
Article Snippet: .. The ST medium consisted of DMEM/Ham’s F-12 (#11581-15, Nacalai Tesque) supplemented with 0.1 mM 2-mercaptoethanol, 0.3% bovine serum albumin (BSA; #01863-77, Nacalai Tesque), 1% insulin–transferrin–selenium–ethanolamine (ITS-X supplement; #094–06761, FUJIFILM Wako), 4% serum replacement (KnockOut Serum Replacement; #10828010, Thermo Fisher Scientific), 2 μM forskolin (#11018, Cayman Chemical), and 2.5 mM Y-27,632, as previously described [ ]. ..



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